New Antibiotic Susceptibility Method Available Through UKVDL

The University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) recently announced the availability of a new antibiotic susceptibility method (the Broth Microdilution Method for determining if bacteria are susceptible to an antimicrobial drug), which provides reproducible test results with minimum inhibitory concentration (MIC) levels (the lowest concentration of an antimicrobial that will inhibit visible growth of a microorganism after overnight incubation).

This state-of-the-art method is endorsed by the American Association of Veterinary Laboratory Diagnosticians and the Clinical and Laboratory Standards Institute. Broth Microdilution Method (or MIC method) includes a broader and updated number of antimicrobials for equine, companion animals, food animals, and poultry. For example, the equine panel includes the drugs amikacin, ampicillin, azithromycin, cefazolin, ceftazidime, ceftiofur, chloramphenicol, clarithromycin, doxycycline, enrofloxacin, erythromycin, gentamicin, imipenem, oxacillin + 2% NaCl, penicillin, rifampin, tetracycline, ticarcillin, ticarcillin/clavulanic acid, and trimethoprim/ sulfamethoxazole.

This method also can determine antimicrobial susceptibility patterns for nocardioform and other slow-growing bacteria, some fungi (yeasts), and some anaerobic bacteria that cannot be assessed by other methods (i.e. Kirby-Bauer disk diffusion).

The MIC method will be the default for every bacterial isolate recovered from clinical specimens unless otherwise indicated by the practitioner. Necropsy cases are still capped and won't be affected by these new test fees. Initial pricing for UKVDL's antibiotic sensitivity testing is as follows:

  • Companion animal, $12/isolate
  • Food animal (including poultry), $6/isolate
  • Mastitis, $6/isolate
  • Horse, $9/isolate
  • Nocardioform, $20/isolate
  • Fungal, $35/isolate

Clients are asked to send specimens in leak-proof containers with enough chill packs to keep them cool and to indicate culture and susceptibility on the submission forms.

Erdal Erol, DVM, PhD, head of diagnostic microbiology at the UKVDL, provided this information.

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